Study of immunogenicity of chlorogenic acid-BSA with different number of CGA / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 2756-2759, 2010.
Artigo
em Chinês
| WPRIM
| ID: wpr-279358
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the immunogenicity of chlorogenic acid-BSA(CGA-BSA) and the foundation for the allergization of CGA-BSA.</p><p><b>METHOD</b>The CGA-BSA with different number of CGA was synthesized to allergize the BALB/c mice and SD rats to get antiserum. The level of IgE and histamine was analyzed by ELISA, and the passive cutaneous anaphylaxis (PCA) test was carried to analyze the antibody titer.</p><p><b>RESULT</b>When the coupling rate of CGA-BSA is 20, the highest IgE and histamine level in BALB/c mice and the antibody titer in PCA are the highest.</p><p><b>CONCLUSION</b>It has better immunogenicity when the coupling rate of CGA-BSA is between 10 to 20, while the CGA-BSA 20 with the best immunogenicity.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Sangue
/
Imunoglobulina E
/
Soroalbumina Bovina
/
Ensaio de Imunoadsorção Enzimática
/
Anafilaxia Cutânea Passiva
/
Histamina
/
Ácido Clorogênico
/
Ratos Sprague-Dawley
/
Alergia e Imunologia
/
Camundongos Endogâmicos BALB C
Limite:
Animais
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2010
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS