Effect of lyophilized powder of Sini powder on 5-HT content and 5-HT(1A) receptor in drosophila heads / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To unveil the mechanism of sleep-improving effect of lyophilized powder of Sini powder on drosophila.</p><p><b>METHOD</b>Drosophila melanogaster whose strain were wild Canton S, unmated, were collected daring 12 h after their emergencing, then they were anaesthetized by small flow of CO2 and divided into different tubes due to their sex. Both male and female flies were distributed randomly into three groups as blank, control and administration, 32 flies each group. The content of 5-HT in the brain was estimated was estimated with ELISA and had a comparison of the expression of 5-HT(1A) receptor in brain, using real-time quantitative PCR.</p><p><b>RESULT</b>Concentration of 5-HT in brain was increased in administration group, and had a significant difference to control group. The expression of 5-HT(1A) receptor was also up-regulated after administrated of Sini powder.</p><p><b>CONCLUSION</b>Sleep-improving effect of Sini powder on drosophila has a contribution to the increasing of 5-HT and 5-HT(1A) receptor in brain.</p>