Astragalus injection ameliorates cisplatin-induced nephrotoxicity in mice / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 2736-2740, 2010.
Artigo
em Chinês
| WPRIM
| ID: wpr-279363
ABSTRACT
<p><b>OBJECTIVE</b>To examine the protective effects of Astragalus injection (AI) on cisplatin (DDP)-induced nephrotoxicity and to explore the mechanism of its nephroprotection.</p><p><b>METHOD</b>The S180-bearing model was established, and fifty mice were randomly divided into five groups control mice, DDP-injected mice (3.5 mg x kg(-1)) and AI-pretreated mice (12, 8, 4 g x kg(-1)). Tumor inhibition rate, renal functions, histological findings were investigated. The apoptotic cells were counted by TUNEL and the presence of Bax, Bcl-2 in renal tissues was determined by immunohistochemistry.</p><p><b>RESULTS</b>Administration of DDP or DDP plus AI showed promising anti-tumor activities as compared with control group. Interestingly, a combination of DDP and AI (12, 8 g x kg(-1)) led to enhanced tumor growth inhibition as compared with DDP alone (all P < 0.05). Compared with that from control, the number of TUNEL-positive cells and the Bax/Bcl-2 ratio was increased significantly in the kidneys of DDP treated mice (P < 0.05). High-dose AI pretreatment significantly reduced the elevated number of TUNEL-positive cells and the Bax/Bcl-2 ratio (P < 0.05), and it showed a superior nephroprotective effect than any other dose. AI significantly also decreased both the damage to renal function and histological pathology.</p><p><b>CONCLUSION</b>Pre-treatment with AI attenuates cisplatin-induced nephrotoxicity without compromising the anti-tumor efficacy of DDP, which might be involved in regulating the Bax and Bcl-2 expression.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Cisplatino
/
Proteínas Proto-Oncogênicas c-bcl-2
/
Astrágalo
/
Tratamento Farmacológico
/
Proteína X Associada a bcl-2
/
Toxicidade
/
Injeções
/
Rim
/
Fitoterapia
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2010
Tipo de documento:
Artigo
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