Metabolites in rat urine after orally administrating gossypetin-8-O-beta-D-glucuronide / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 2585-2589, 2010.
Artigo
em Chinês
| WPRIM
| ID: wpr-279395
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the metabolites in rat urine after orally administrating gossypetin-8-O-beta-D-glucuronide.</p><p><b>METHOD</b>Gossypetin-8-O-beta-D-glucuronide was administrated orally at the dose of 250 mg x kg(-1) to healthy rats, then the urine was collected, pre-treated appropriately and detected by HPLC-DAD-MS(n).</p><p><b>RESULT</b>In the rat urine, gossypetin-8-O-beta-D-glucuronide, along with 6 metabolites, were detected, and their structures were elucidated on the basis of reference standards, UV and MS(n) spectra. The metabolic pathways of gossypetin-8-O-beta-D-glucuronide included mainly deglycosylation, glucuronidation and methylation.</p><p><b>CONCLUSION</b>A rapid and sensitive method for the analysis of metabolites in rat urine after orally administrating gossypetin-8-O-beta-D-glucuronide was established and this will be useful for the further metabolism study of gossypetin-8-O-beta-D-glucuronide.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Flavonoides
/
Espectroscopia de Ressonância Magnética
/
Administração Oral
/
Cromatografia Líquida de Alta Pressão
/
Ratos Sprague-Dawley
/
Metabolismo
Limite:
Animais
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2010
Tipo de documento:
Artigo
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