Determination of 25(R, S) ruscogenin 1-O-beta-D-glucopyranosyl (1 --> 2) beta-D-xylopyranosyl (1 --> 3) beta-D-fucopyranoside in Liriope muscari from different habitats and different harvest time by HPLC-ELSD / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 2508-2510, 2010.
Artigo
em Chinês
| WPRIM
| ID: wpr-279411
ABSTRACT
<p><b>OBJECTIVE</b>To develop an HPLC-ELSD method for the determination of 25 (R, S) ruscogenin 1-O-[beta-D-glucopyranosyl (1 --> 2)] [beta-D-xylopyranosyl (1 --> 3)] beta-D-fucopyranoside in the tuberous roots of Liriope muscari from different habitats and different harvest time.</p><p><b>METHOD</b>A Shimadzu C18 column (4.6 mm x 150 mm, 5 microm) with a solvent system consisting of acetonirile-water (46 54) was used, and detected by ELSD. The temperature of drift tube was 94 degrees C and the nebulizer nitrogen flow rate was 2.5 L x min(-1).</p><p><b>RESULT</b>The calibration curve of 25 (R, S) ruscogenin 1-O-[beta-D-glucopyranosyl (1 --> 2)] [beta-D-xylopyranosyl (1 --> 3)] beta-D-fucopyranoside showed good linearity in the range of 1.02-12.228 microg and the average recovery was 100.80%, with RSD of 1.8%. 10 batches of L. muscari from different habitats were analyzed, and the contents were 0.25% - 0.41%. The contents of 15 batches from different harvest time were 0.13%-0.38%.</p><p><b>CONCLUSION</b>The method is simple, rapid and sensitive, and can be used for determination of 25 (R, S) ruscogenin 1-O-[beta-D-glucopyranosyl (1 --> 2)] [beta-D-xylopyranosyl (1 --> 3)] beta-D-fucopyranoside in L. muscari. It provides the valuable basis for quality assessment of L. muscari.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Fisiologia
/
Saponinas
/
Espirostanos
/
Triterpenos
/
Dados de Sequência Molecular
/
Espectroscopia de Ressonância Magnética
/
Estrutura Molecular
/
Química
/
Cromatografia Líquida de Alta Pressão
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2010
Tipo de documento:
Artigo
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