Simultaneous determination of five flavonoid compounds in leaves of Adinandra nitida by HPLC-PAD / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 2406-2409, 2010.
Artigo
em Chinês
| WPRIM
| ID: wpr-279432
ABSTRACT
<p><b>OBJECTIVE</b>To established a novel HPLC-PAD method for the simultaneous determination of five compounds (camellianin A, camellianin B, apigenin, quercitrin, epicatechin) in leaves of Adinandra nitida.</p><p><b>METHOD</b>These compounds were effectively separated on a reversed-phase C18 column (4.6 mm x 250 mm, 10 microm) with the column temperature at 25 degrees C. The mobile phase was composed of 0.1% aqueous formic acid and methanol. The flow rate was 1.0 mL x min(-1), the detection wavelength was set at 280 nm before 15 min for detecting epicatechin and at 262 nm from 15 to 40 min for detecting the rest four compounds.</p><p><b>RESULT</b>All the linearities were good (r > 0.9991) within their test ranges. The established method showed good precision with overall intra-day and interday RSD values less than 3.33% and 3.2%, respectively. The average recoveries were in the range of 96.08% to 100.30% with RSD from 2.0% to 4.0%. The limits of detection (LOD) and quantification (LOQ) in the range of ranged from 0.9 to 5.6 ng and 3.0 to 19.0 ng, respectively.</p><p><b>CONCLUSION</b>This established method can be applied to evaluate the intrinsic quality of the leaves of A. nitida.</p>
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DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Flavonoides
/
Medicamentos de Ervas Chinesas
/
Química
/
Cromatografia Líquida de Alta Pressão
/
Folhas de Planta
/
Magnoliopsida
/
Métodos
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2010
Tipo de documento:
Artigo
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