Dynamic expression of matrix metalloproteinase-2, membrane type-matrix metalloproteinase-2 in experimental hepatic fibrosis and its reversal in rat / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 328-331, 2004.
Artigo
em Chinês
| WPRIM
| ID: wpr-279543
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression dynamics and significance of matrix metalloproteinase-2 (MMP-2) membrane type-matrix metalloproteinase-2 (MT-MMP-2) in hepatic fibrosis and its reversal counterpart.</p><p><b>METHODS</b>An experimental CCl4 induced hepatic fibrosis rat model was established by intraperitoneal administration of carbon tetrachloride for 2, 4, 6, 8, 10 weeks, and normal rats were used as a control group. The immunohistochemical methods and in situ hybridization were used to detect MMP-2,MT-MMP-2 mRNA and related antigens in the liver.</p><p><b>RESULTS</b>MMP-2,MT-MMP-2 mRNA and related antigens were expressed in mesenchymal cells and parts of hepatocytes besides active pathological changes, especially in the fibrous septum and portal area. Expression of MMP-2,MT-MMP-2 mRNA and related antigens were increased in hepatic fibrosis and decreased gradually in its reversal counterpart.</p><p><b>CONCLUSION</b>This study suggested that mesenchymal cells are the main cellular origins of MMPs. The levels of MMP-2 and MT-MMP-2 antigens and gene expression were closely related to hepatic fibrosis. MMP-2 and MT-MMP-2 may play important roles in hepatic fibrosis and its reversal counterpart.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
RNA Mensageiro
/
Intoxicação por Tetracloreto de Carbono
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Regulação Enzimológica da Expressão Gênica
/
Ratos Wistar
/
Metaloproteinase 2 da Matriz
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Metaloproteinases da Matriz
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Hepatócitos
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Metaloproteinases da Matriz Associadas à Membrana
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Células-Tronco Mesenquimais
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Experimental and Clinical Virology
Ano de publicação:
2004
Tipo de documento:
Artigo
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