Capturing-ELISA for the detection of anti-HCMV-IgM antibody in serum / 中华实验和临床病毒学杂志

ABSTRACT

<p><b>BACKGROUND</b>To develop a capturing-ELISA for the detection of anti-HCMV-IgM antibody in serum.</p><p><b>METHODS</b>The anti-HCMV-IgM antibody was detected in 68 patients with HCMV infection by the capturing-ELISA, and the results were compared with those of indirect ELISA.</p><p><b>RESULTS</b>The specificity and sensitivity of the capturing-ELISA were shown to be significantly higher than those of indirect ELISA, and its results were not affected by RF factor.</p><p><b>CONCLUSION</b>The capturing ELISA is specific, sensitive, convenient and reliable method which may be feasible for clinical use.</p>