The cloning and sequencing of H-2Kk gene cDNA of 615 mice / 华西口腔医学杂志
West China Journal of Stomatology
;
(6): 313-315, 2002.
Artigo
em Chinês
| WPRIM
| ID: wpr-279624
ABSTRACT
<p><b>OBJECTIVE</b>The purposes of this study were to clone and sequence the major histocompatibility complex type I (MHC I) molecular antigen recognizing gene (H-2Kk) of 615 mice, and to provide the functional gene for transgenic therapy.</p><p><b>METHODS</b>The 1.4 kb full-length fragment of H-2Kk gene complementary DNA (cDNA) was amplified from the total RNA of 615 mouse liver by using reverse transcription polymerase chain reaction (RT-PCR). The cDNA was inserted into PGEM3Zf(+) vector directionally, and the competent E. coli JM109 was transformed with the ligated product. The recombinant PGEM3Zf(+)-H-2Kk cDNA plasmid was obtained using restricted enzyme analysis of the transfectants. The complete sequence of 615 mouse H-2Kk cDNA was determined by using Sanger's method.</p><p><b>RESULTS</b>The sequences of 615 mouse H-2Kk cDNA were 99% similar with those of H-2Kk cDNA which were reported by other researchers, and the sequences encoding antigen recognizing regions (ARS) were identical with each other.</p><p><b>CONCLUSION</b>The authors cloned the MHC I molecular antigen recognizing gene (H-2Kk) of 615 mice successfully and got the functional gene of MHC I.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Genes MHC Classe I
/
Terapia Genética
/
Antígenos H-2
/
Clonagem Molecular
/
Análise de Sequência de DNA
/
Mutação Puntual
/
DNA Complementar
/
Transgenes
/
Escherichia coli
/
Genes
Limite:
Animais
Idioma:
Chinês
Revista:
West China Journal of Stomatology
Ano de publicação:
2002
Tipo de documento:
Artigo
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