The Usefulness of Calretinin Immunohistochemistry for Rectal Suction Biopsy to Diagnose Hirschsprung’s Disease / 소아외과
Journal of the Korean Association of Pediatric Surgeons
;
: 23-28, 2016.
Artigo
em Coreano
| WPRIM
| ID: wpr-27976
ABSTRACT
PURPOSE:
Hirschsprung’s disease (HD) is a congenital intestinal disorder with absence of ganglion cells in the intestinal muscle and submucosa. Diagnosis is based on histopathological study such as H&E, and acetylcholinesterase (AchE) immunohistochemistry. Calretinin immunohistochemistry was introduced as a new diagnostic method against limitations of other staining. The aim of this study is to investigate the usefulness of calretinin immunohistochemistry for the diagnosis of HD compared to H&E and AchE.METHODS:
Ten patients with HD and 22 non-HD patients were included in the study. H&E staining, AchE and calretinin immunohistochemistry were performed in all 32 patients. All slides were evaluated by same single pathologist and the diagnostic value was calculated for each H&E stain, AchE immunohistochemical staining, and calretinin immunohistochemical staining.RESULTS:
Calretinin method had sensitivity of 100% and specificity of 100% for diagnosis of HD. Its diagnostic accuracy was 100%. AchE staining showed 100% of specificity and 80% of sensitivity. Diagnostic accuracy of H&E staining was 56.3%.CONCLUSION:
We concluded that calretinin immunohistochemistry is a very useful and valuable method to diagnosis HD patient.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Acetilcolinesterase
/
Sucção
/
Biópsia
/
Imuno-Histoquímica
/
Sensibilidade e Especificidade
/
Cistos Glanglionares
/
Diagnóstico
/
Calbindina 2
/
Hematoxilina
/
Doença de Hirschsprung
Tipo de estudo:
Estudo diagnóstico
Limite:
Humanos
Idioma:
Coreano
Revista:
Journal of the Korean Association of Pediatric Surgeons
Ano de publicação:
2016
Tipo de documento:
Artigo
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