Detection of fusion genes resulting from chromosome abnormalities in childhood acute lymphoblastic leukemia / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 551-553, 2005.
Artigo
em Chinês
| WPRIM
| ID: wpr-280004
ABSTRACT
<p><b>OBJECTIVE</b>To detect the expression of the fusion genes resulting from chromosome abnormalities in childhood acute lymphoblastic leukemia(ALL) and its conformity to WHO classification.</p><p><b>METHODS</b>Sixty-two children with ALL were investigated. The expression of fusion genes was determined by multiplex reverse transcription-polymerase chain reaction (RT-PCR), karyotyping (R band) and immunophenotyping (by flow cytometry) were also performed.</p><p><b>RESULTS</b>Of the 62 patients, 23(37.1%) were found to carry 13 different fusion genes. The patients with immunophenotype of Pre-B-ALL were found to carry TEL/AML1(3 cases); E2A/PBX1, E2A/HLF, TLS/ERG, MLL/AF4, MLL/AF9, MLL/AF10, MLL/AFX-MLL/AF6-MLL/ELL, MLL/AF6-MLL/ELL, dupMLL (one case for each); and HOX11 (6 cases). The patients with immunophenotype of Pre-T-ALL were found to carry TAL1D (4 cases, one is also found to have HOX11 expression); and HOX11 (2 cases). The multiplex RT-PCR in combination with chromosome analysis revealed genetic abnormalities in 69.4%(43/62) of childhood ALL.</p><p><b>CONCLUSION</b>Multiplex RT-PCR combined with chromosome analysis and immunophenotyping can provide reliable and helpful information for the diagnosis, therapy evaluation and prognosis prediction in childhood ALL, which may also serve as a basis on which to implement the criteria of WHO classification.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fatores de Transcrição
/
Proteínas de Fusão Oncogênica
/
Imunofenotipagem
/
Aberrações Cromossômicas
/
Proteínas Proto-Oncogênicas
/
Proteínas de Homeodomínio
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Proteína FUS de Ligação a RNA
/
Proteínas de Ligação a DNA
/
Proteína de Leucina Linfoide-Mieloide
Tipo de estudo:
Estudo diagnóstico
/
Estudo prognóstico
Limite:
Adolescente
/
Criança
/
Criança, pré-escolar
/
Humanos
/
Lactente
Idioma:
Chinês
Revista:
Chinese Journal of Medical Genetics
Ano de publicação:
2005
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS