Cloning of full-length cDNA sequence of a novel candidate gene related to mouse cleft palate / 中华医学遗传学杂志

ABSTRACT

<p><b>OBJECTIVE</b>Cloning and screening a novel candidate gene related to developing mouse cleft palate.</p><p><b>METHODS</b>The differentially expressed genes were cloned by modified PCR-based subtractive hybridization. After identification using reverse dot blotting, positive clones were sequenced and analyzed for homology in GenBank databases.</p><p><b>RESULTS</b>Four novel express sequence tags were obtained, one of which spanning 809 bp was the full-length of the novel gene cDNA identified by Northern hybridization.</p><p><b>CONCLUSION</b>A novel candidate gene related to mouse cleft palate was cloned.</p>