Effect of caspase 9 related signaling molecules on the apoptosis of human vascular endothelial cell induced by homocysteine / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 383-386, 2005.
Artigo
em Inglês
| WPRIM
| ID: wpr-280045
ABSTRACT
<p><b>OBJECTIVE</b>To understand the role of mitochondria associated signaling pathway in the apoptosis of human vascular endothelial cell induced by homocysteine (Hcy).</p><p><b>METHODS</b>The mRNA and protein expression levels of the up-stream signaling molecules of caspase 3, Bcl 2, caspase 9, and cytosolic cytochrome-c, were investigated. The in vitro cultured human umbilical vein endothelial cells with homocysteine at different concentrations were incubated for 24 h. The expressions of Bcl 2 and caspase 9 at mRNA and protein levels were analyzed by reverse transcription-polymerase chain reaction(RT-PCR) and Western blot. Cytochrome-c in cytoplasm was also detected by Western blot.</p><p><b>RESULTS</b>The expression levels of three signaling molecules were all down-regulated by homocysteine at both mRNA and protein levels in a dose-dependent manner.</p><p><b>CONCLUSION</b>Homocysteine could affect the formation of apoptosome through repressing the expression of Bcl 2 gene and release of cytochrome-c from mitochondria. Decreasing of apoptosome could disturb the activation of caspase 9. The results also indicate that the mitochondria pathway is not the major signaling pathway involved in Hcy-induced apoptosis.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Transdução de Sinais
/
Células Cultivadas
/
Western Blotting
/
Apoptose
/
Proteínas Proto-Oncogênicas c-bcl-2
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Biologia Celular
/
Células Endoteliais
/
Citocromos c
Limite:
Humanos
Idioma:
Inglês
Revista:
Chinese Journal of Medical Genetics
Ano de publicação:
2005
Tipo de documento:
Artigo
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