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Association of glucocoid receptor binding activity and heat shock protein expressions after acute lung injury in mice / 南方医科大学学报
Journal of Southern Medical University ; (12): 725-728, 2008.
Artigo em Chinês | WPRIM | ID: wpr-280110
ABSTRACT
<p><b>OBJECTIVE</b>To study the association between decreased ligand binding activity of glucocoid receptor (GR) and heat shock protein 90 (Hsp90), the molecular chaperone of GR, after acute lung injury (ALI) in mice.</p><p><b>METHODS</b>In mouse models of oleic acid-induced ALI, the levels of GR, Hsp90 and Hsp70 were dynamically observed using Western blotting, and the binding capacity and binding affinity of GR assessed with radioligand binding assay.</p><p><b>RESULTS</b>After ALI, pulmonary edema was significantly aggravated in the mice with significantly increased lung body index and lung water ratio. GR increased within 1 h after the injury, but then decreased significantly to the lowest level at 12 h after the injury, and the levels of Hsp90 and Hsp70 was increased obviously and reached the highest at 12 h. Radioligand binding assay showed that the Bmax decreased gradually and Kd value increased, and these changes were consistent with the changes of Hsp90/GR.</p><p><b>CONCLUSION</b>The ligand binding activity of GR is related to the changes of Hsp90 after ALI.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Ensaio Radioligante / Sítios de Ligação / Distribuição Aleatória / Receptores de Glucocorticoides / Proteínas de Choque Térmico HSP90 / Proteínas de Choque Térmico HSP70 / Ácido Oleico / Lesão Pulmonar Aguda / Metabolismo Limite: Animais Idioma: Chinês Revista: Journal of Southern Medical University Ano de publicação: 2008 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Ensaio Radioligante / Sítios de Ligação / Distribuição Aleatória / Receptores de Glucocorticoides / Proteínas de Choque Térmico HSP90 / Proteínas de Choque Térmico HSP70 / Ácido Oleico / Lesão Pulmonar Aguda / Metabolismo Limite: Animais Idioma: Chinês Revista: Journal of Southern Medical University Ano de publicação: 2008 Tipo de documento: Artigo