Fluorescent linear labelling method for biochip applications / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 405-412, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-280190
ABSTRACT
Fluorescent Linear Labelling technique is an effective, single-tube and linear amplification method. The sample cDNA was synthesized from total RNA, and was labelled antisense cRNA from double-stranded cDNA with T7RNA polymerase; it can be used for hybridization to oligonucleotide biochip. Fluorescent Linear Labelling Method can result in 50- to 100-fold higher degrees of amplification, and has been shown to retain information on transcript abundance, thus making it an efficient, robust technique for fluorescent labelling on biochip.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Coloração e Rotulagem
/
Química
/
Análise de Sequência com Séries de Oligonucleotídeos
/
Corantes Fluorescentes
/
Métodos
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Biomedical Engineering
Ano de publicação:
2009
Tipo de documento:
Artigo
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