Antimicrobial activities of the culture supernatant of 293 cells transfected with recombinant HBD-2 gene eukyriotic expressive plasmid / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 371-373, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-280197
ABSTRACT
In this study, HBD-2 eukaryotic expression recombinant plasmid pcDNA 3.1-zeo(+)-HBD-2 was constructed by the molecular cloning technologies, and 293 cells were transfected by liposome. The cell culture supernatant was collected and its antibacterial activities against Staphylococcus aureus ATCC25923 and Pseudomonas aeruginosa ATCC27853 were tested. The antibacterial activity against S. aureus of the culture supernatant of the HBD-2 gene transfected-293 cells was detected, whereas in the same condition, the antibacterial activity against P. aeruginosa was not detected. This result suggested that HBD-2 may preferentially kill Staphylococcus aureus.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Plasmídeos
/
Proteínas Recombinantes
/
Transfecção
/
Linhagem Celular
/
Embriologia
/
Beta-Defensinas
/
Biologia Celular
/
Vetores Genéticos
/
Genética
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Biomedical Engineering
Ano de publicação:
2009
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS