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Effects of cell-mediated immunity induced by intramuscular chitosan-pJME/ GM-CSF nano-DNA vaccine in BAlb/c mice / 病毒学报
Chinese Journal of Virology ; (6): 423-428, 2014.
Artigo em Chinês | WPRIM | ID: wpr-280348
ABSTRACT
This study aimed to investigate the immune adjuvant effect and mechanism induced by chitosan nanoparticles carrying pJME/GM-CSF. In this study, plasmid DNA (pJME/GM-CSF) was encapsulated in chitosan to prepare chitosan-pJME/GM-CSF nanoparticles using a complex coacervation process. Immunohistochemistry was used to detect the type of infiltrating cells at the site of intramuscular injection. The phenotype and functional changes of splenic DCs were measured by flow cytometry after different immunogens were injected intramuscularly. The killing activity of CTLs was assessed using the lactate dehydrogenase (LDH) release assay. The preparation of chitosan-pJME/GM-CSF nanoparticles matched the expected theoretical results. Our results also found that, after pJME/GM-CSF injection, the incoming cells were a mixture of macrophages, neutrophils, and immature DCs. Meanwhile, pJME/GM-CSF increased the expression of MHC class II molecules on splenic DCs, and enhanced their Ag capture and presentation functions. Cell-mediated immunity was induced by the vaccine. Furthermore, chitosan-pJME/GM-CSF nanoparticles outperformed the administration of standard pJME/GM-CSF in terms of DC recruitment, antigen processing and presentation, and vaccine enhancement. These findings reveal that chitosan could be used as delivery vector for DNA vaccine intramuscular immunizations, and enhance pJME/GM-CSF-induced cellular immune responses.
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Baço / Virologia / Células Dendríticas / Linfócitos T Citotóxicos / Adjuvantes Imunológicos / Fator Estimulador de Colônias de Granulócitos e Macrófagos / Encefalite Japonesa / Vacinas de DNA / Vacinas contra Encefalite Japonesa / Quitosana Limite: Animais / Feminino / Humanos Idioma: Chinês Revista: Chinese Journal of Virology Ano de publicação: 2014 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Baço / Virologia / Células Dendríticas / Linfócitos T Citotóxicos / Adjuvantes Imunológicos / Fator Estimulador de Colônias de Granulócitos e Macrófagos / Encefalite Japonesa / Vacinas de DNA / Vacinas contra Encefalite Japonesa / Quitosana Limite: Animais / Feminino / Humanos Idioma: Chinês Revista: Chinese Journal of Virology Ano de publicação: 2014 Tipo de documento: Artigo