Active immunotherapy of allergic asthma with a recombinant human interleukin-5 protein as vaccine in a murine model / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 1517-1522, 2007.
Artigo
em Inglês
| WPRIM
| ID: wpr-280395
ABSTRACT
<p><b>BACKGROUND</b>Eosinophils are highly related to allergic asthma inflammation. Interleukin (IL)-5 is the major chemokine of eosinophils, inhibition of the activity of IL-5 thus seems to be a potential approach to asthma therapy. The current study was performed to determine whether a recombinant human IL-5 protein as a xenogeneic vaccine has the capability of inducing anti-asthma activities.</p><p><b>METHODS</b>Recombinant human IL-5 was used as a protein vaccine. Mouse asthma model was established to observe the anti-asthma activities. Lung histology was observed; eosinophils in blood and bronchoalveolar lavage were stained and counted. Airway hyperresponsiveness was determined by whole body plethysmograph. Antibody characters and cytokines were detected with enzyme linked immunosorbent assay (ELISA) and Western blot assay.</p><p><b>RESULTS</b>Vaccination with recombinant human IL-5 protein as vaccine significantly reduced airway inflammation and airway hyperresponsiveness, and shifted the cytokine production from Th2 (IL-4) to Th1 (INF-gamma) in mice allergic-asthma model. Immunization with recombinant human IL-5 protein vaccine bypassed the immunological tolerance and induced production of polyclonal antibodies that were cross-reactive with murine IL-5.</p><p><b>CONCLUSIONS</b>Active immunization with xenogeneic homologous IL-5 may be a possible therapeutic approach to the treatment of asthma and potentially of other eosinophilic disorders.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Asma
/
Terapêutica
/
Proteínas Recombinantes
/
Linfócitos B
/
Citocinas
/
Interleucina-5
/
Vacinação
/
Hiper-Reatividade Brônquica
/
Células Th2
/
Modelos Animais de Doenças
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Inglês
Revista:
Chinese Medical Journal
Ano de publicação:
2007
Tipo de documento:
Artigo
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