Detection of pim-1 mRNA in prostate cancer diagnosis / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 1491-1493, 2007.
Artigo
em Inglês
| WPRIM
| ID: wpr-280400
ABSTRACT
<p><b>BACKGROUND</b>Pim-1 plays an important role in the apoptosis, proliferation, differentiation of cancer cells and progression of cancer. In this study we detected the expression of pim-1 mRNA in normal prostate, benign prostatic hyperplasia (BPH), and prostate cancer (PCa) and explored its diagnostic value for PCa.</p><p><b>METHODS</b>The prostate tissues were collected from 23 patients with PCa, 37 patients with BPH, and 3 healthy volunteers. Pim-1 mRNA expression levels in these samples were determined by the quantitative real-time PCR (QRT-PCR). The differences of expression were calculated based on a standard curve.</p><p><b>RESULTS</b>The ratio of pim-1 mRNA to beta-actin in the normal prostate, BPH, and PCa were 1.05 +/- 0.04, 2.57 +/- 0.74 and 4.45 +/-0.63, respectively. The differences among PCa, BPH and NT were significant (P < 0.05, respectively).</p><p><b>CONCLUSION</b>Detecting pim-1 mRNA expression by QRT-PCR provides a reliable metric for the diagnosis of PCa.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Próstata
/
Hiperplasia Prostática
/
Neoplasias da Próstata
/
RNA Mensageiro
/
Reação em Cadeia da Polimerase
/
Sensibilidade e Especificidade
/
Diagnóstico
/
Proteínas Proto-Oncogênicas c-pim-1
/
Genética
/
Metabolismo
Tipo de estudo:
Estudo diagnóstico
Limite:
Idoso
/
Humanos
/
Masculino
Idioma:
Inglês
Revista:
Chinese Medical Journal
Ano de publicação:
2007
Tipo de documento:
Artigo
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