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Analysis of 187 children with enteroviral central nervous system infection in Shandong area / 中华儿科杂志
Chinese Journal of Pediatrics ; (12): 199-202, 2003.
Artigo em Chinês | WPRIM | ID: wpr-280539
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the diagnostic potential of previously published enterovirus (EV) reverse transcription polymerase chain reaction (RT-PCR) assay in detection of EV in CSF samples from children with a diagnosis of aseptic meningitis and to investigate the clinical characteristics of the patients seen in Shandong.</p><p><b>METHODS</b>EV RNA was detected in 187 CSF samples and serum and/or urine samples of a part of patients by RT-PCR and viral culture technique.</p><p><b>RESULTS</b>RT-PCR was positive in all 62 CSF specimens which were positive by cell culture (100%). In addition, 93 of 125 (74.4%) CSF samples negative by cell culture were RT-PCR positive. In 4 of these 93 (4.3%) patients, viral culture of specimens from other sites (serum or urine) was also positive. The sensitivity of CSF RT-PCR based on clinical diagnosis in patients with meningitis of negative bacterial culture results was 82.9% (155/187), which was considerably higher than the sensitivity of CSF virus culture 33.2% (62/187). The results of RT-PCR can be reported within 4 hours, whereas the viral culture of CSF requires 4.6 days for a cytopathic effect to develop. EV meningitis occurred in a sporadic form and in some areas there were outbreaks. The clinical characteristics of 155 patients with EV meningitis were different in different age groups.</p><p><b>CONCLUSION</b>EV was one of the most common causes of aseptic meningitis in Shandong area. The RT-PCR assay was rapid, sensitive and specific for the diagnosis of EV meningitis and may be a potential tests to shorten hospital stay and reduce the use of antibiotics.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Urina / Sangue / RNA Viral / Células HeLa / Infecções do Sistema Nervoso Central / Líquido Cefalorraquidiano / China / Enterovirus / Reação em Cadeia da Polimerase Via Transcriptase Reversa / Diagnóstico Tipo de estudo: Estudo diagnóstico Limite: Criança / Criança, pré-escolar / Feminino / Humanos / Lactente / Masculino / Recém-Nascido País/Região como assunto: Ásia Idioma: Chinês Revista: Chinese Journal of Pediatrics Ano de publicação: 2003 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Urina / Sangue / RNA Viral / Células HeLa / Infecções do Sistema Nervoso Central / Líquido Cefalorraquidiano / China / Enterovirus / Reação em Cadeia da Polimerase Via Transcriptase Reversa / Diagnóstico Tipo de estudo: Estudo diagnóstico Limite: Criança / Criança, pré-escolar / Feminino / Humanos / Lactente / Masculino / Recém-Nascido País/Região como assunto: Ásia Idioma: Chinês Revista: Chinese Journal of Pediatrics Ano de publicação: 2003 Tipo de documento: Artigo