Detection of expression of Th1/Th2 cytokines in jurkat cell treated with gamma-interferon by cytometric bead array / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 356-358, 2006.
Artigo
em Chinês
| WPRIM
| ID: wpr-280667
ABSTRACT
In order to explore the value of gamma-interferon (IFN-gamma) in therapy of T lymphoid leukemia and role of cytometric bead array (CBA) method in detection of Th1/Th2 cytokines expression, the Jurkat cells were cultured for 48 hours in different concentration of IFN-gamma, the expressions of IL-2, IL-6, INF-gamma and TNF-alpha in culture supernatants were assayed by CBA method; CD25 expression was assayed by flow cytometry. The results showed that the expressions of IL-2, TNF-alpha were enhanced in INF-gamma dose-dependent way, and the expression of CD25 was also enhanced, but there was no expression of IL-6. It is concluded that Jurkat cells induced by IFN-gamma were able to express high-level of Th1 cytokine and IL-2 membrane receptor (CD25), and the CBA method can be used to exactly evaluate the dynamic change of Th1/Th2 cytokines.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Ensaio de Imunoadsorção Enzimática
/
Sensibilidade e Especificidade
/
Interferon gama
/
Interleucina-2
/
Fator de Necrose Tumoral alfa
/
Células Th2
/
Células Th1
/
Células Jurkat
/
Citometria de Fluxo
Tipo de estudo:
Estudo diagnóstico
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Experimental Hematology
Ano de publicação:
2006
Tipo de documento:
Artigo
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