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Effect of diallyl disulfide on expression and secretion of VEGF in HL-60 leukemic cells / 中国实验血液学杂志
Journal of Experimental Hematology ; (6): 212-216, 2006.
Artigo em Chinês | WPRIM | ID: wpr-280699
ABSTRACT
The study was aimed to investigate the expression of VEGF mRNA and VEGF protein in HL-60 cells treated with diallyl disulfide (DADS), and to explore the antileukemic mechanism of DADS in respect of VEGF production. Semi-quantitative RT-PCR and ELISA were used to detect the expression of VEGF mRNA and secretion of VEGF protein in HL-60 cell lines treated by DADS respectively. The results showed that the expression of VEGF mRNA and secretion of VEGF protein were found in HL-60 cells. The expression of VEGF mRNA and secretion of VEGF protein in HL-60 cells could be down regulated by treatment with 0.625, 1.25, and 2.5 microg/mL DADS for 48 and 72 hours and the effects had a dose dependent relationship (r > 0.9, P < 0.01). The differences between DADS treated HL-60 cell groups and the control group were statistically significant (P < 0.01), there were also statistically significant differences among three DADS-treated HL-60 cell groups (P < 0.05). It is concluded that DADS effectively inhibits the proliferation of human leukemia cell line HL-60 cells; DADS exerts its antileukemic effects by reduction of the expression of VEGF mRNA and VEGF protein secretion.
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Farmacologia / RNA Mensageiro / Células HL-60 / Fator A de Crescimento do Endotélio Vascular / Proliferação de Células / Dissulfetos / Compostos Alílicos / Genética / Antineoplásicos Limite: Humanos Idioma: Chinês Revista: Journal of Experimental Hematology Ano de publicação: 2006 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Farmacologia / RNA Mensageiro / Células HL-60 / Fator A de Crescimento do Endotélio Vascular / Proliferação de Células / Dissulfetos / Compostos Alílicos / Genética / Antineoplásicos Limite: Humanos Idioma: Chinês Revista: Journal of Experimental Hematology Ano de publicação: 2006 Tipo de documento: Artigo