Detection of complex karyotype in a myelodysplastic syndrome cell line (MUTZ-1) by metaphase fluorescence in situ hybridization / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 46-49, 2006.
Artigo
em Inglês
| WPRIM
| ID: wpr-280736
ABSTRACT
This study was aimed to investigate the cytogenetic changes of MDS cell line (MUTZ-1) with chromosome 5q deletion. R-banding analysis was used to identify chromosome aberrations in MDS cell line and Vysis Spectra Vysion M-FISH was used to further characterize chromosomal complex karyotype. The results indicated that M-FISH exhibited obvious chromosomal aberrations with high frequency including translocation, insertion, breakage and rearrangement, deletion and increasement of chromosome number, the complex karyotype of MUTZ-1 was shown as 50, xx, der (1) t (1;2), ins (1;14), +der (2) t(2;19), der (3) t (3;5), der (3) (3522), 5q-, der (6) t (3;6), der (7) (18717), +8, +der (9) t (1;9), der (10) t (1;10), +11, +12, der (?13) (101358), der (14) t (8;14), der (14) t (14, 15), der (15) t (15;21) x 2, +17, +18, -21, -22. It is concluded that M-FISH analysis revealed obvious changes in complex karyotype of MDS cell line MUTZ-1, and the M-FISH technique can increase accuracy of detection for chromosomal complex karyotype, and help diagnosis and prognostic evaluation of MDS.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Translocação Genética
/
Síndromes Mielodisplásicas
/
Cromossomos Humanos Par 5
/
Células Tumorais Cultivadas
/
Aberrações Cromossômicas
/
Deleção Cromossômica
/
Hibridização in Situ Fluorescente
/
Genética
/
Cariotipagem
/
Métodos
Tipo de estudo:
Estudo diagnóstico
Limite:
Criança, pré-escolar
/
Feminino
/
Humanos
Idioma:
Inglês
Revista:
Journal of Experimental Hematology
Ano de publicação:
2006
Tipo de documento:
Artigo
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