Lack of telomerase activity in rabbit bone marrow stromal cells during differentiation along neural pathway / 中华创伤杂志(英文版)
Chinese Journal of Traumatology
; (6): 201-205, 2006.
Article
em En
| WPRIM
| ID: wpr-280911
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate telomerase activity in rabbit bone marrow stromal cells (BMSCs) during their committed differentiation in vitro along neural pathway and the effect of glial cell line-derived neurotrophic factor (GDNF) on the expression of telomerase.</p><p><b>METHODS</b>BMSCs were acquired from rabbit marrow and divided into control group, GDNF (10 ng/ml) group. Cytokine.NSCs medium (prepared by our lab, Patent No. ZL02134314. 4) supplemented with 10 percent fetal bovine serum (FBS) was used to induce BMSCs differentiation along neural pathway. Fluorescent immunocytochemistry was employed to identify the expressions of Nestin, neuron-specific endase (NSE), and gial fibrillary acidic protein (GFAP). The growth curves of the cells and the status of cell cycles were analyzed, respectively. During the differentiation, telomerase activities were detected using the telomeric repeat amplification protocol-enzyme-linked immunosorbent assay (TRAP-ELISA).</p><p><b>RESULTS</b>BMSCs were successfully induced to differentiate along neural pathway and expressed specific markers of fetal neural epithelium, mature neuron and glial cells. Telomerase activities were undetectable in BMSCs during differentiation along neural pathway. Similar changes of cell growth curves, cell cycle status and telomerase expression were observed in the two groups.</p><p><b>CONCLUSIONS</b>Rabbit BMSCs do not display telomerase activity during differentiation along neural pathway. GDNF shows little impact on proliferation and telomerase activity of BMSCs.</p>
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Índice:
WPRIM
Assunto principal:
Células da Medula Óssea
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Imuno-Histoquímica
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Diferenciação Celular
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Células Estromais
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Telomerase
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Fator Neurotrófico Derivado de Linhagem de Célula Glial
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Metabolismo
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Chinese Journal of Traumatology
Ano de publicação:
2006
Tipo de documento:
Article