Simultaneous determination of three active compounds in root barks of Dictamnus dasycarpus by RP-HPLC / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To develop a RP-HPLC method for simultaneous determination of three active compounds, dictamnine, obacunone and fraxinellone in root bark of Dictamnus dasycarpus and supply a reference for the establishment of the quality standard of D. dasycarpus.</p><p><b>METHOD</b>A Kromasil C18 column was used with methanol-water (60:40) as the mobile phase, at the flow rate of 1 mL x min(-1). 236 nm was selected as the detected wavelength.</p><p><b>RESULT</b>The determined three compounds were well separated with a linear range of 0.0021-0.1060, 0.0201-0.9200 and 0.0102-1.020 g x L(-1), respectively. The recoveries of them were 100.5%, 99.2% and 100.2%, respectively.</p><p><b>CONCLUSION</b>This method is simple, rapid and accurate, particularly suitable for the quality control of D. dasycarpus.</p>