Expression of HSP27 in colorectal carcinoma and its relationship with lymphatic metastasis / 南方医科大学学报
Journal of Southern Medical University
;
(12): 41-44, 2008.
Artigo
em Chinês
| WPRIM
| ID: wpr-281486
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the relationship between heat shock protein 27 (HSP27) expression and lymphatic metastasis of colorectal carcinoma (CRC).</p><p><b>METHODS</b>Immunohistochemistry was used to detect HSP27 expression in 68 specimens of human CRC. The expression of HSP27 mRNA and protein was also detected in two colorectal carcinoma cell lines with different lymphatic metastasis potentials by RT-PCR, Western blotting and immunohistochemistry.</p><p><b>RESULTS</b>HSP27 expression was not related to such clinicopathological factors as the patient's age, gender, histological grade, lymphatic metastasis or clinical stages (P>0.05), but overexpression of HSP27 showed significant inverse correlation to lymphatic metastasis of CRC (P=0.035). HSP27 mRNA and protein were expressed at low levels in SW620 cells with high potentials for lymphatic metastasis, and at high levels in SW480 cells that had low lymphatic metastasis potentials.</p><p><b>CONCLUSION</b>HSP27 expression can be inversely correlated to metastatic behavior of CRC cells, and may play a role in suppressing the metastasis of CRC.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
RNA Mensageiro
/
Imuno-Histoquímica
/
Neoplasias Colorretais
/
Adenocarcinoma
/
Regulação Neoplásica da Expressão Gênica
/
Western Blotting
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Linhagem Celular Tumoral
/
Proteínas de Choque Térmico HSP27
Limite:
Adulto
/
Idoso
/
Aged80
/
Feminino
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2008
Tipo de documento:
Artigo
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