Rearrangement of immunoglobulin heavy chain variable region genes in human neonates / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1901-1904, 2007.
Artigo
em Chinês
| WPRIM
| ID: wpr-281509
ABSTRACT
<p><b>OBJECTIVE</b>To study the rearrangement of immunoglobulin (Ig) heavy chain variable region (V(H)) genes in human neonates with different gestational ages (GA).</p><p><b>METHODS</b>Peripheral blood from the neonates with GA of 27 weeks (4 cases), 28-32 weeks (9 cases), 33-36 weeks (12 cases), and 37-42 weeks (13 cases) was collected. RT-PCR was used to amplify the Ig V(H) gene, and the PCR products were separated by electrophoresis and analyzed using 6% denaturing PAGE gel.</p><p><b>RESULTS</b>All Ig V(H) family genes had several rearranged genes in each GA group, and the neonates with different GA showed no significant difference in the median molecular weight for each rearranged Ig V(H) family gene.</p><p><b>CONCLUSION</b>The neonates with GA of 27-42 weeks exhibit diversity in Ig V(H) gene rearrangement, and for the same Ig V(H) family, the median length of the arranged Ig V(H) genes is independent of the gestational age.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Região Variável de Imunoglobulina
/
Rearranjo Gênico
/
Família Multigênica
/
Idade Gestacional
/
Cadeias Pesadas de Imunoglobulinas
/
Análise de Sequência de DNA
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Genes de Cadeia Pesada de Imunoglobulina
/
Genética
Limite:
Humanos
/
Recém-Nascido
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2007
Tipo de documento:
Artigo
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