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Primary cultured neonate rat sinus node cells: morphology and expression of kir2.1 protein / 南方医科大学学报
Journal of Southern Medical University ; (12): 1701-1705, 2007.
Artigo em Chinês | WPRIM | ID: wpr-281558
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression of kir2.1 protein in primary cultured sinus node cells and establish a reliable technique to locate, culture and characterize neonatal rat sinus node cells.</p><p><b>METHODS</b>In paraffin sections, the location and morphology of the neonatal rat sinus node cells were observed by HE staining, silver nitrate staining, myelin staining and phosphotungstic acid-hematoxylin (PTAH) staining. Primary cell culture from the neonatal rat sinus node was conducted to observe the spontaneous contraction frequency, cell morphology and kir2.1 protein expression.</p><p><b>RESULTS</b>Combination of the 3 staining methods allowed accurate localization of the sino-atrial nodal (SAN) tissue, and among the cultured cells in the SAN, at least 3 distinct types of cells with spontaneous contraction were observed. The majority of the contracting cells were spindle cells and their construction and impulse frequency indicated their identity as pacemaker cells, while the triangular and irregular cells resembled the atrial muscle cells. A lower expression level of kir2.1 protein was detected in SAN cells than in the atrial and ventricular myocytes of the neonatal rats.</p><p><b>CONCLUSION</b>Combination of silver nitrate staining, myelin staining and PTAH staining identifies the exact location of the sinus node tissue, and cultured sinus node cells have lower expression of kir2.1 protein than the atrial and ventricular myocytes of neonatal rats.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Nó Sinoatrial / Células Cultivadas / Ratos Sprague-Dawley / Técnicas de Cultura de Células / Biologia Celular / Canais de Potássio Corretores do Fluxo de Internalização / Metabolismo / Métodos Limite: Animais Idioma: Chinês Revista: Journal of Southern Medical University Ano de publicação: 2007 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Nó Sinoatrial / Células Cultivadas / Ratos Sprague-Dawley / Técnicas de Cultura de Células / Biologia Celular / Canais de Potássio Corretores do Fluxo de Internalização / Metabolismo / Métodos Limite: Animais Idioma: Chinês Revista: Journal of Southern Medical University Ano de publicação: 2007 Tipo de documento: Artigo