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STI571 enhances the effect of arsenic trioxide and Velcade on bcr/abl+-CD34+ cell proliferation and apoptosis / 南方医科大学学报
Journal of Southern Medical University ; (12): 1665-1669, 2007.
Artigo em Chinês | WPRIM | ID: wpr-281567
ABSTRACT
<p><b>OBJECTIVE</b>To study the effects of STI571, arsenic trioxide (As2O3) and Velcade, used alone or in combination, on the proliferation and apoptosis of bcr/abl+-CD34+ cells.</p><p><b>METHODS</b>bcr/abl+-CD34+ cells isolated from the bone marrow of patients with chronic myeloid leukemia (CML) were treated for 96 h with STI571, As2O3 and Velcade either alone and in combination, and the cell proliferation and apoptosis were analyzed by CCK-8 assay and flow cytometry. The morphological changes of the apoptotic cells were observed by Hoechst33342 staining and fluorescent microscope. The inhibitory effects of the drugs on normal CD34+ cells were also observed.</p><p><b>RESULTS</b>Low-concentration STI571, As2O3 or Velcade all dose-dependently inhibited bcr/abl+-CD34+ cell proliferation without obvious apoptosis-inducing effects. STI571 at 0.25-2 micromol/L combined with As2O3 at 2.5 micromol/L and with Velcade at 15 nmol/L both significantly increased the cell inhibition and apoptosis rates, showing obvious additive or synergistic effects of the drugs without further enhancement of normal CD34+ cell inhibition.</p><p><b>CONCLUSION</b>Combination with STI571 enhances the effects of As2O3 and Velcade on bcr/abl+-CD34+ cells, suggesting the potential clinical value of this regimen.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Óxidos / Patologia / Farmacologia / Piperazinas / Arsenicais / Pirazinas / Pirimidinas / Benzamidas / Ácidos Borônicos / Células Tumorais Cultivadas Limite: Adulto / Idoso / Humanos Idioma: Chinês Revista: Journal of Southern Medical University Ano de publicação: 2007 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Óxidos / Patologia / Farmacologia / Piperazinas / Arsenicais / Pirazinas / Pirimidinas / Benzamidas / Ácidos Borônicos / Células Tumorais Cultivadas Limite: Adulto / Idoso / Humanos Idioma: Chinês Revista: Journal of Southern Medical University Ano de publicação: 2007 Tipo de documento: Artigo