Maxillary sinus floor augmentation using gene-enhanced tissue engineered bone / 中华口腔医学杂志
Chinese Journal of Stomatology
;
(12): 114-117, 2012.
Artigo
em Chinês
| WPRIM
| ID: wpr-281651
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the effect of maxillary sinus elevation with gene-enhanced tissue engineering bone in dogs.</p><p><b>METHODS</b>bone marrow stromal cells (BMSC) derived from dog marrow were cultured, and transduced with the adenovirus carrying bone morphogenetic protein-2 (BMP-2) gene (AdBMP-2), the adenovirus carrying green fluorescent protein (GFP) gene (AdGFP) in vitro. The bone formation ability of gene modified BMSC with scaffold was examined in nude mice and in elevated maxillary sinus of dog. Student's t-test was used for statistical analysis with SPSS 11.0 software package.</p><p><b>RESULTS</b>Gene transfection efficiency reached up to (83.95 ± 2.43)% as demonstrated by GFP expression. Ectopic bone formation was detected in nude mice. As for maxillary sinus floor elevation in a dog model, new bone formation area in the AdBMP-2 gene transduced BMSC with Bio-Oss group was significantly higher than in BMSC with Bio-Oss group at 120 d (P < 0.05).</p><p><b>CONCLUSIONS</b>AdBMP-2 gene transduced BMSC can stimulate ectopic bone formation in nude mice, and promote bone formation and maturation in the dog maxillary sinus.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Osteogênese
/
Transplante
/
Matriz Óssea
/
Proteínas Recombinantes
/
Transfecção
/
Células Cultivadas
/
Adenoviridae
/
Transplante Ósseo
/
Biologia Celular
/
Engenharia Tecidual
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Stomatology
Ano de publicação:
2012
Tipo de documento:
Artigo
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