Determination of tomatine in Solanum cathayanum by RP-HPLC / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 882-884, 2010.
Artigo
em Chinês
| WPRIM
| ID: wpr-281695
ABSTRACT
<p><b>OBJECTIVE</b>To develop an HPLC method for determination of tomatine in the dried Solanum cathayanum of China Hubei Enshi.</p><p><b>METHOD</b>The analysis was performed on a YMC-Pack ODS-AA column (4. 6 mm x 150 mm, 5 microm) eluted with acetonitrile and water in gradient mode. The concentration of acetonitrile in the mobile phase changes from 20% to 100% within 60 minutes. The detection wavelength was set 203 nm. The flow rate was 1.0 mL x min(-1) and column temperature was set at 30 degrees C.</p><p><b>RESULT</b>The linear relationship of tomatine was determined within the range from 0.1-0.6 g x L(-1) (r = 0.999 7). The average recovery as 98.93% with RSD 1.2%.</p><p><b>CONCLUSION</b>A convenient and reliable method was developed to determine the content of tomatine in the dried S. cathayanum.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Tomatina
/
Química
/
Cromatografia Líquida de Alta Pressão
/
Solanum
/
Cromatografia de Fase Reversa
/
Métodos
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2010
Tipo de documento:
Artigo
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