Establishing DNA chip technique for detecting hepatitis C virus genotypes and primary application / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 375-377, 2003.
Article
em Zh
| WPRIM
| ID: wpr-281778
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish a new method based DNA chip technique for detecting HCV genotypes.</p><p><b>METHODS</b>Genotyping probes were designed according to the sequence of HCV 5' NCR to generate DNA chip. The probes on DNA chip contains 5 major genotypes and 8 subtypes. The DNA fragment amplified by labeling Cy5 fluorescence was hybridized with DNA chip.</p><p><b>RESULTS</b>Fifty-five out of 65 isolates detected by DNA chip belonged to 1b- DNA sequencing of form a part of the isolates was used as the control. The results of both were completely consistent.</p><p><b>CONCLUSION</b>The method is simple and rapid with high specificity and sensitivity. It can be applied in detection of HCV RNA and genotypes.</p>
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Sangue
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RNA Viral
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Reação em Cadeia da Polimerase
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Sensibilidade e Especificidade
/
Classificação
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Hepacivirus
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Regiões 5' não Traduzidas
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Análise de Sequência com Séries de Oligonucleotídeos
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Genética
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Genótipo
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Experimental and Clinical Virology
Ano de publicação:
2003
Tipo de documento:
Article