Construction of replication-deficient recombinant adenovirus expressing gag-polDelta and gp140TM genes of human immunodeficiency virus in mice / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 150-153, 2004.
Artigo
em Chinês
| WPRIM
| ID: wpr-281831
ABSTRACT
<p><b>BACKGROUND</b>Construction of replication-deficient recombinant adenovirus expressing gag-pol and env genes of human immunodeficiency virus (HIV) in mice.</p><p><b>METHODS</b>gag-polDelta and gp140TM genes were cloned into shuttle vector pAdTrack-CMV respectively, and then the plasmids containing gag-polDelta or gp140TM gene were cotransformed with the backbone of adenovirus into E.coli BJ5183. Transfections of the recombinants were performed to obtain recombinant adenoviruses. Its immunogenicity was evaluated by testing antibody levels of mice primed with DNA vaccines and boosted with recombinant adenoviruses.</p><p><b>RESULTS</b>The replication-deficient recombinant adenovirus could express Gp140TM, Gag P55 and P24 proteins correctly. The mice primed with DNA vaccines and boosted with recombinant adenoviruses elicited high titer of HIV-1-specific antibody compared with that inoculated with DNA vaccines only.</p><p><b>CONCLUSION</b>Replication-deficient recombinant adenovirus expressing gag-polDelta and gp140TM can elicit high titer HIV-1-specific antibodies.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Recombinação Genética
/
Transfecção
/
Produtos do Gene env
/
Adenoviridae
/
Proteínas de Fusão gag-pol
/
HIV-1
/
Vacinas contra a AIDS
/
Vacinas de DNA
/
Alergia e Imunologia
/
Produtos do Gene env do Vírus da Imunodeficiência Humana
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Experimental and Clinical Virology
Ano de publicação:
2004
Tipo de documento:
Artigo
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