Heterogeneity of HIV strains isolated from different tissues of 3 AIDS patients / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 58-61, 2003.
Artigo
em Chinês
| WPRIM
| ID: wpr-281854
ABSTRACT
<p><b>BACKGROUND</b>To reveal the characteristics of genotype and phenotype of HIV strains in blood and some tissues of AIDS patients.</p><p><b>METHODS</b>The virus was isolated from peripheral blood mononuclear cell (PBMC),cerebrospinal fluid (CSF)and lymph nodes of 3 AIDS patients by coculture with PBMC stimulated by PHA for 72 hours from uninfected donor. The cytopathic effect of the HIV isolates was determined in cultured MT2 cell line. The env gene sequences form proviral DNA were analyzed by GCG software.</p><p><b>RESULTS</b>In one patient,there were differences between the strains from blood and different tissues both in genotype and phenotype. The biological phenotypes of two strains from CSF were non syncytium (NSI) type, their env sequences were similar to standard CNS tropic strain (SF162).</p><p><b>CONCLUSIONS</b>The viral heterogeneity exists in different body compartments within an infected individual. The neurotropic isolate which is similar to international standard strain exists in some AIDS patients in China.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fenótipo
/
Virologia
/
Leucócitos Mononucleares
/
Síndrome da Imunodeficiência Adquirida
/
HIV
/
Heterogeneidade Genética
/
Técnicas de Cocultura
/
Genótipo
/
Linfonodos
Limite:
Adulto
/
Feminino
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
Chinese Journal of Experimental and Clinical Virology
Ano de publicação:
2003
Tipo de documento:
Artigo
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