Quantitative analysis of HBV DNA amplified products with microtiter hybridization / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 39-41, 2003.
Article
em Zh
| WPRIM
| ID: wpr-281859
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>BACKGROUND</b>To establish a new assay for detecting the quantity of HBV DNA with PCR and enzyme-linked immunosorbent assay(ELISA).</p><p><b>METHODS</b>The products of PCR using primers pre-labeled with biotin were hybridized with the capture probes that were immobilized on the microtiter strips and then bond with Sav-Ap. The quantity of DNA was detected by measuring the yellow color at 405 nm wave length.</p><p><b>RESULTS</b>Totally 125 sera from patients with hepatitis B were tested for HBV DNA by this method,the sera were also tested for HBV immunological markers by solid phase radio immuno-assay (SPRIA). The HBV positive rate with PCR-ELISA was 64.9% (24/37) in samples which were positive for HBsAg, HBeAg and HBcAb; and 34.2% (13/38) in sera which were positive for HBsAg, HBeAb and HBcAb; in sera positive for HBsAg and HBcAb or only HBcAb, the positive rate was 6.7% (1/15) and 5.9% (2/34) respectively.</p><p><b>CONCLUSIONS</b>The PCR ELISA assay is simple and suitable for clinical laboratory in quantitative determination of HBV DNA.</p>
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Índice:
WPRIM
Assunto principal:
Virologia
/
Sangue
/
DNA Viral
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Ensaio de Imunoadsorção Enzimática
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Sondas de DNA
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Vírus da Hepatite B
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Reação em Cadeia da Polimerase
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Sensibilidade e Especificidade
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Genética
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Hepatite B
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Experimental and Clinical Virology
Ano de publicação:
2003
Tipo de documento:
Article