Sequence analysis of the deletion and mutation in carboxy terminal region of the Epstein-Barr virus latent membrane protein 1 derived from nasopharyngeal carcinoma patients / 中华实验和临床病毒学杂志

ABSTRACT

<p><b>BACKGROUND</b>To study the deletion and mutation in carboxy terminal region of LMP1 gene derived from nasopharyngeal carcinoma (NPC) in Guangdong and Guangxi, the high risk areas of nasopharyngeal carcinoma in China.</p><p><b>METHODS</b>LMP1 gene carboxy terminal region was amplified from nasopharyngeal carcinoma tissues by PCR, and then cloned and sequenced.</p><p><b>RESULTS</b>Of the 20 cases, 17 were LMP1 positive. In all positive cases, only 1 case did not show deletion. Four positive cases were chosen for DNA sequencing, The rusult showed that all the four cases had mutation and the 30bp deletion.</p><p><b>CONCLUSIONS</b>High frequency of deletion and mutation in LMP1 gene of nasopharyngeal carcinoma tissues was found in Guangdong and Guangxi. Whether it related to the high incidence of NPC should be further studied.</p>