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Antigenic analysis of the common recombinant mutant types of HBsAg / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology ; (6): 31-34, 2003.
Artigo em Chinês | WPRIM | ID: wpr-281861
ABSTRACT
<p><b>BACKGROUND</b>To study the difference between the mutant types and the wild type of HBsAg on the binding efficiency with antibodies in order to find some methods to detect the mutants.</p><p><b>METHODS</b>The recombinant wild type of HBsAg and the mutants including 145 R, 133 T, 126 S, 141 E, 126 S+133 T, 126 S+133 T+145 R and 126 S+133 T+141 E were cloned, respectively. Then the expression vector containing the wild or mutant gene was transfected into COS7 cells, and the recombinant proteins were expressed. The proteins were detected with the routine HBsAg kits.</p><p><b>RESULTS</b>The binding efficiency with monoclonal antibodies of HBsAg expressed by 126 S was higher than that of the wild type, while the results of 145 R, 141 E, 126 S+133 T+145 R and 126 S+133 T+141 E were lower than that of the wild, and 133 T was similar to the wild. But most of the mutants had the same reactivity with the polyclonal antibody.</p><p><b>CONCLUSIONS</b>The effect of mutations on antibody binding differs depending on the amino acid involved and on the location within the "a" loop. So it is necessary to use polyclonal antibody or to find new kits to detect the mutants of HBsAg.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Sítios de Ligação de Anticorpos / Proteínas Recombinantes / Vírus da Hepatite B / Técnicas de Transferência de Genes / Alergia e Imunologia / Vetores Genéticos / Genética / Antígenos de Superfície da Hepatite B / Mutação / Epitopos Limite: Humanos Idioma: Chinês Revista: Chinese Journal of Experimental and Clinical Virology Ano de publicação: 2003 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Sítios de Ligação de Anticorpos / Proteínas Recombinantes / Vírus da Hepatite B / Técnicas de Transferência de Genes / Alergia e Imunologia / Vetores Genéticos / Genética / Antígenos de Superfície da Hepatite B / Mutação / Epitopos Limite: Humanos Idioma: Chinês Revista: Chinese Journal of Experimental and Clinical Virology Ano de publicação: 2003 Tipo de documento: Artigo