In vitro anti-angiogenic action of lambda-carrageenan oligosaccharides / 药学学报
Acta Pharmaceutica Sinica
; (12): 595-600, 2007.
Article
em Zh
| WPRIM
| ID: wpr-281870
Biblioteca responsável:
WPRO
ABSTRACT
This study was designed to evaluate the inhibition effect of lambda-carrageenan oligosaccharides on neovascularization in vitro by chick chorioallantoic membrane (CAM) model and human umbilical vein endothelial cell ( HUVEC). lambda-Carrageenan oligosaccharides caused a dose-dependent decrease of the vascular density of CAM, and adversely affected capillary plexus formation. At a high concentration of 1 mg x mL(-1), this compound inhibited the endothelial cell proliferation, while low concentration of lambda-carrageenan oligosaccharides (< 250 microg x mL(-1)) affected the cell survival slightly (> 95%). Different cytotoxic sensitivity of lambda-carrageenan oligosaccharides in three kinds of cells was observed, of which HUVEC is the most sensitive to this oligosaccharides. The inhibitory action of lambda-carrageenan oligosaccharides on the endothelial cell invasion and migration was also observed at relatively low concentration (150 - 300 microg x mL(-1)) through down-regulation of intracellular matrix metalloproteinases-2 (MMP-2) expression on endothelial cells. Current observations demonstrated that lambda-carrageenan oligosaccharides are potential angiogenesis inhibitor with combined effects of inhibiting invasion, migration and proliferation.
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Oligossacarídeos
/
Farmacologia
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Carragenina
/
Movimento Celular
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Metaloproteinase 2 da Matriz
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Inibidores da Angiogênese
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Biologia Celular
/
Células Endoteliais
/
Membrana Corioalantoide
/
Proliferação de Células
Tipo de estudo:
Prognostic_studies
Limite:
Animals
/
Humans
Idioma:
Zh
Revista:
Acta Pharmaceutica Sinica
Ano de publicação:
2007
Tipo de documento:
Article