Protective effect of total glucosides of Picrorhiza scrophulariiflora against oxidative stress in glomerular mesangial cells induced by high glucose / 药学学报
Acta Pharmaceutica Sinica
; (12): 381-385, 2007.
Article
em Zh
| WPRIM
| ID: wpr-281889
Biblioteca responsável:
WPRO
ABSTRACT
Total glucosides of Picrorhiza scrophulariiflora (TGP) is the active principal in Picrorhiza scrophulariiflora which has antioxidant effect. Since oxidative stress plays a key role in diabetic nephropathy, we investigated the effects of TGP on oxidative stress in bovine glomerular mesangial cells (MC) induced by prolonged high glucose. Bovine glomerular mesangial cells were cultured and passages 2-3 were used for the experiment. Mesangial cells were cultured in high glucose medium, and treated with TGP for 3 weeks. Then collagen IV excreted by mesangial cells were detected, and the percentages of cell cycle were observed by flow cytometry technique. The levels of reactive oxygen species (ROS), mitochondrial membrane potential (MMP) and [Ca2+]i were measured by flow cytometry after loaded with fluorescent probe DCFH-DA, Rh123 and fluo-3-acetoxymethylest. TGP significantly decreased the excretion of collagen IV and cell hypertrophy induced by high glucose, reduced the levels of ROS and [Ca2+]i, and increased MMP. Therefore we conclude that TGP could protect mesangial cells against oxidative stress induced by high glucose.
Texto completo:
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Índice:
WPRIM
Assunto principal:
Farmacologia
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Plantas Medicinais
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Ciclo Celular
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Células Cultivadas
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Química
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Cálcio
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Espécies Reativas de Oxigênio
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Estresse Oxidativo
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Secreções Corporais
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Biologia Celular
Limite:
Animals
Idioma:
Zh
Revista:
Acta Pharmaceutica Sinica
Ano de publicação:
2007
Tipo de documento:
Article