Pharmacokinetics of PEG-rhG-CSF and rHSA-hG-CSF in mice determined with ELISA / 药学学报
Acta Pharmaceutica Sinica
;
(12): 197-200, 2007.
Artigo
em Chinês
| WPRIM
| ID: wpr-281942
ABSTRACT
Double antibody sandwich-type ELISA was used to detect rhG-CSF in serum to study the pharmacokinetics of rhG-CSF, PEG-rhG-CSF and rHSA-hG-CSF in mice and to confirm that PEGlyation and albumin fusion of rhG-CSF technology can prolong half-life of G-CSF. Pharmacokinetic parameters were calculated with 3P87 software. T1/2 s of rhG-CSF, PEG-rhG-CSF and rHSA-hG-CSF are 2. 1 , 14.2 and 10. 6 h, respectively. T1/2 s of PEG- rhG-CSF and rHSA-hG-CSF are 7, 5 times than T1/2 s of rhG-CSF, respectively. Tpeak s of PEG-rhG-CSF and rHSA-hG-CSF are 15, 13 times than Tpeak of rhG-CSF, respectively. The result of ELISA indicates that PEGlyation and albumin fusion of rhG-CSF technology can prolong half-life of G-CSF.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Polietilenoglicóis
/
Sangue
/
Proteínas Recombinantes de Fusão
/
Proteínas Recombinantes
/
Albumina Sérica
/
Ensaio de Imunoadsorção Enzimática
/
Farmacocinética
/
Química
/
Fator Estimulador de Colônias de Granulócitos
/
Área Sob a Curva
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
Acta Pharmaceutica Sinica
Ano de publicação:
2007
Tipo de documento:
Artigo
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