Comparison of sonoporation effect of liposomes and phospholipids-based microbubbles on cultured cell membrane / 药学学报
Acta Pharmaceutica Sinica
; (12): 1176-1179, 2006.
Article
em Zh
| WPRIM
| ID: wpr-281965
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WPRO
ABSTRACT
<p><b>AIM</b>To compare sonoporation effect of two phospholipids-based vectors-liposomes and microbubbles on cultured cell membrane.</p><p><b>METHODS</b>A breast cancer cell line SK-BR-3 was exposed to ultrasound alone, 2% or 5% liposome + ultrasound and 2% or 5% microbubble + ultrasound, separately. Immediately after the experiment and 24 h after ultrasound exposure, atomic-force microscopy (AFM) scanning was used to observe the membrane change of SK-BR-3 cells.</p><p><b>RESULTS</b>After ultrasound exposure, normal SK-BR-3 cells more or less lost their natural shape, showing elliptic outline with obtuse curved boundary. In groups added with phospholipids-based microbubbles, more obtuse curved boundary of cells was observed. The membrane pores of SK-BR-3 cells had apparent changes after ultrasound exposure. With AFM technique, membrane pores under ultrasound alone or ultrasound with liposomes conditions were enlarged, the diameter of some pores exceeding 1 microm. But all the membrane pores in these conditions returned to normal appearance after 24 hours. In ultrasound with 2% microbubble condition, most membrane pores were about 1 - 3 microm in size and returned to normal appearance after 24 h. In ultrasound with 5% microbubble condition, however, pores of most cell membrane porosity was about 2 - 4 pm and did not totally return to normal appearance after 24 h.</p><p><b>CONCLUSION</b>At 2% concentration, phospholipids-based microbubble could enhance ultrasonic sonoporation effect and produce reparable membrane pores on SK-BR-3 cells, which appeared to be a promising vehicle for drug and gene delivery.</p>
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Índice:
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Assunto principal:
Fosfolipídeos
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Sonicação
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Portadores de Fármacos
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Permeabilidade da Membrana Celular
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Química
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Porosidade
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Tecnologia Farmacêutica
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Microbolhas
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Lipossomos
Idioma:
Zh
Revista:
Acta Pharmaceutica Sinica
Ano de publicação:
2006
Tipo de documento:
Article