A novel erythroid differentiation related gene EDRF1 upregulating globin gene expression in HEL cells / 中华医学杂志(英文版)
Chinese Medical Journal
; (24): 1701-1705, 2002.
Article
em En
| WPRIM
| ID: wpr-282105
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To further characterize the differentiation inducing properties of EDRF1 and demonstrate its functional pathway involved in regulation of globin gene expression.</p><p><b>METHODS</b>By transfecting EDRF1 sense and antisense constructs into HEL cells, we identified the expression of globin and erythropoietin receptor genes by Northern blot analysis. RT-PCR and EMSA (electrophoresis mobility shift assay) were performed to monitor the expression and DNA-binding activity of erythroid specific transcription factors GATA-1 and NF-E2.</p><p><b>RESULTS</b>It was shown that when EDRF1 was overexpressed, production of alpha-globin increased. In antisense EDRF1, overexpression of HEL cells, significant loss of alpha-, gamma-globin mRNA synthesis was observed. The transcription of endogenous GATA-1 and NF-E2 mRNA expression were maintained at the same levels compared with control experiments. However, the transcription activity of GATA-1 was severely impaired. Expression of erythropoietin receptor gene was not influenced by EDRF1 gene overexpression.</p><p><b>CONCLUSION</b>The results suggested that EDRF1 regulated alpha- and gamma-globin gene synthesis by modulating DNA-binding activity of GATA-1 transcription factor.</p>
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Fisiologia
/
Fatores de Transcrição
/
Globinas
/
RNA Mensageiro
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Diferenciação Celular
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Regulação para Cima
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Células Cultivadas
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Regulação da Expressão Gênica
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Proteínas de Ligação a DNA
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Eritropoese
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
Chinese Medical Journal
Ano de publicação:
2002
Tipo de documento:
Article