Detecting K-ras and p53 gene mutation from stool and pancreatic juice for diagnosis of early pancreatic cancer / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 1632-1636, 2002.
Artigo
em Inglês
| WPRIM
| ID: wpr-282121
ABSTRACT
<p><b>OBJECTIVE</b>To explore new methods for the early diagnosis of pancreatic cancer through detection of K-ras and p53 mutations in pancreatic juice and stool.</p><p><b>METHODS</b>201 patients in PUMC Hospital from 1994 - 2000 and 60 control individuals were enrolled in this study. K-ras point mutation was detected by PCR-RFLP while p53 mutation was detected by PCR-SSCP.</p><p><b>RESULTS</b>K-ras mutation was found in pancreatic juice in 87.8% (36/41) of pancreatic cancer patients and 23.5% (4/17) of benign pancreatic disease patients. In 261 stool specimens, amplification found mutations successfully in 235 patients (90%). K-ras mutation was found in stool in 88% (66/75) of pancreatic cancer patients, 51.1% (24/47) of benign pancreatic disease patients and 19.6% (9/46) of normal individuals. p53 mutation was found in pancreatic juice in 47.4% (18/38) of pancreatic cancer patients and 12.5% (2/16) of benign pancreatic disease patients. p53 mutation was found in stool in 37.1% (23/62) and 19.1% (4/21) of chronic pancreatitis patients.</p><p><b>CONCLUSION</b>K-ras mutation in pancreatic juice has higher diagnosis sensitivity and specificity, and therefore may be used as a supplement in the diagnosis of pancreatic cancer. Detection of K-ras mutation combined with p53 mutation in stool can aid in the screening of pancreatic cancer.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Suco Pancreático
/
Neoplasias Pancreáticas
/
Polimorfismo de Fragmento de Restrição
/
Química
/
Reação em Cadeia da Polimerase
/
Genes p53
/
Genes ras
/
Polimorfismo Conformacional de Fita Simples
/
Diagnóstico
/
Fezes
Tipo de estudo:
Estudo diagnóstico
/
Estudo de rastreamento
Limite:
Humanos
Idioma:
Inglês
Revista:
Chinese Medical Journal
Ano de publicação:
2002
Tipo de documento:
Artigo
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