Difference in DNA sequences in SSU rDNA variable regions among pathogens isolated from different epidemic foci of visceral leishmaniasis in China / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 1457-1459, 2002.
Artigo
em Inglês
| WPRIM
| ID: wpr-282165
ABSTRACT
<p><b>OBJECTIVE</b>To confirm the existence of point mutations in the SSU rDNA variable regions of 5 Leishmania donovani (L.d.) isolates from different epidemic foci in China.</p><p><b>METHODS</b>Specific SSU rDNA fragments from nuclear DNA of 7 Leishmania species/isolates were amplified by PCR and then cloned into pGEM(R)-T Easy Vectors. After that, the specific fragments were sequenced by an automated DNA sequencer.</p><p><b>RESULTS</b>Sequence analysis showed that the amplified DNA fragments of 7 Leishmania species/isolates were all 392 bp in length. All 5 point mutations were located in two unique sequence blocks (UQ-I and UQ-II), and no insertions or deletions were found. The identities of comparison of Leishmania in GeneBank were more than 98%.</p><p><b>CONCLUSION</b>Five point mutations exist in the SSU rDNA variable region of 5 L.d. isolates from different epidemic foci of visceral leishmaniasis (VL) in China. Sequence differences of the SSU rDNA variable region exist among L.d. isolates from different foci.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Parasitologia
/
Leishmania donovani
/
DNA Ribossômico
/
Química
/
Reação em Cadeia da Polimerase
/
DNA de Protozoário
/
Mutação Puntual
/
Genética
/
Leishmaniose Visceral
Limite:
Animais
/
Humanos
Idioma:
Inglês
Revista:
Chinese Medical Journal
Ano de publicação:
2002
Tipo de documento:
Artigo
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