Effects of realgar on tissue factor expression of NB4 and MR2 cells / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 553-556, 2003.
Artigo
em Chinês
| WPRIM
| ID: wpr-282269
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of Realgar on procoagulant activity (PCA), tissue factor expression and tissue factor mRNA transcription in acute promyelocytic leukemia (APL) cell lines NB4 and MR2 cells.</p><p><b>METHOD</b>NB4 and MR2 cells were treated with 300 micrograms.L-1 Realgar PCA of the treated cells was detected using one-stage clotting assay. TF antigen was detected by ELISA and TFmRNA by semi-quantitive RT-PCR.</p><p><b>RESULT</b>The PCA and TF antigen level in NB4 and MR2 cells were significantly higher than that in HL-60 and K562 cells. Realgar could down-regulate the membrane PCA, TF antigen and TF mRNA transcription of NB4 and MR2 cells in a time-dependent manner.</p><p><b>CONCLUSION</b>Down-regulating TF expression and PCA of NB4 and MR2 cells by Realgar may be one of the mechanism of its improvement effect on DIC-related hemorrhage of APL patients.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Farmacologia
/
Arsenicais
/
Sulfetos
/
Tretinoína
/
Fatores de Coagulação Sanguínea
/
RNA Mensageiro
/
Tromboplastina
/
Cisteína Endopeptidases
/
Materia Medica
Limite:
Humanos
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2003
Tipo de documento:
Artigo
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