Effects of phytoestrogens on gap junctional intercellular communication / 中华预防医学杂志
Chinese Journal of Preventive Medicine
;
(12): 126-128, 2005.
Artigo
em Chinês
| WPRIM
| ID: wpr-282374
ABSTRACT
<p><b>OBJECTIVE</b>To observe effects of phytoestrogens quercetin (QC), Genistein (GEN), coumestrol (COM), and enterolactone (ENL) on gap junctional intercellular communication (GJIC) in HaCaT cells.</p><p><b>METHODS</b>HaCaT cells were exposed to QC, GEN, COM, and ENL at 0.1, 1.0, 10.0 and 100.0 micromol/L for 24 hours. The effects of phytoestrogens on GJIC were determined by fluorescence redistribution after photobleaching (FRAP) technique of using a laser scanning confocal microscope (LSCM).</p><p><b>RESULTS</b>QC did not affect the GJIC at 0.1-10.0 micromol/L, whereas, GEN, COM, and ENL exhibited inhibition on the GJIC in some extent at 0.1-10.0 micromol/L without showing significant cytotoxicity. The ratio of fluorescence recovery were between 31.77% to 37.06%, which were significantly decreased compared the vehicle control (44.74%).</p><p><b>CONCLUSION</b>The phytoestrogens GEN, COM, and ENL, but not QC, could inhibit the GJIC function in HaCaT cells at concentrations could be reached in human serum in some instance, indicating they could, under certain conditions, be cancer promoters. Therefore, it should be prudent to use these chemicals as pharmaceuticals or dietary supplements.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Fisiologia
/
Quercetina
/
Comunicação Celular
/
Linhagem Celular
/
Junções Comunicantes
/
Microscopia Confocal
/
Genisteína
/
Cumestrol
/
Fitoestrógenos
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Preventive Medicine
Ano de publicação:
2005
Tipo de documento:
Artigo
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