Protective effects of curcumin on vascular endothelial injuries induced by radiation / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases
;
(12): 846-848, 2011.
Artigo
em Chinês
| WPRIM
| ID: wpr-282480
ABSTRACT
<p><b>OBJECTIVE</b>To study the effects of curcumin on vascular endothelial injuries induced by radiation and the mechanism.</p><p><b>METHODS</b>Human umbilical vein endothelial cells (HUVEC) were isolated, cultured and divided into the control group and 4 groups exposed to 3-ray at the doses of 2, 4, 6 and 8 Gy. Cellular morphological and ultrastructural changes were examined under light microscopy and electron microscopy respectively. Flow cytometry was used to detect the cellular apoptosis, necrosis and intracellular reactive oxygen species(ROS) generation. The contents of Lactate dehydrogenase (LDH) and malondialdehyde (MDA) in the cultures were measured before and after irradiation.</p><p><b>RESULTS</b>The results of cellular morphological and ultrastructural ex-aminations shown that the typical apoptotic changes appeared after irradiation. The rates of apoptosis and necrosis in groups pretreated with curcumin were significantly lower than those in other groups (P<0.05). LDH and MDA in the irradiation groups were significantly higher than those in curcumin pretreatment groups (P<0.05). The ROS generation in radiation groups significantly increased with the radiation doses, as compared with the groups pre-treated with curcumin (P<0.05).</p><p><b>CONCLUSION</b>Irradiation induced the apoptosis and necrosis of HUVEC, and increased significantly the intracellular LDH and MDA levels in a dose-dependent. Curcumin had the protective effects on HUVEC from the apoptosis and necrosis induced by radiation.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Efeitos da Radiação
/
Espécies Reativas de Oxigênio
/
Apoptose
/
Curcumina
/
Lesões do Sistema Vascular
/
Células Endoteliais da Veia Umbilical Humana
/
L-Lactato Desidrogenase
/
Malondialdeído
/
Metabolismo
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Industrial Hygiene and Occupational Diseases
Ano de publicação:
2011
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS