Effects of Astragalus Membranaceus Injection on TNF-alpha-induced release of inflammatory factors from HUVECs and the molecular mechanisms / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1137-1140, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-282601
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the protective effect of Astragalus Membranaceus Injection on human umbilical vein endothelial cells (HUVECs) against tumor necrosis factor alpha (TNF-alpha).</p><p><b>METHODS</b>Cultured passage 2 HUVECs were stimulated with TNF-alpha with or without a 2-h Astragalus Membranaceus Injection treatment. The expression of nuclear factor-kappaB (NF-kappaB) subunit p65 were evaluated by immuncytochemistical method, and the levels of p65 in the nuclei and the protein Ikappabetaalpha in the cytoplasm were evaluated by Western blotting. The levels of interleukin-6 (IL-6) and soluble intracellular adhesion molecule-1 (sICAM-1) in the cell culture were determined with ELISA.</p><p><b>RESULTS</b>TNF-alpha induced the activation of NF-kappaB and increased the expressions of IL-6 and sICAM-1 in HUVECs. The activation of NF-kappabeta by TNF-alpha was suppressed by Astragalus Membranaceus Injection in a dose-dependent manner.</p><p><b>CONCLUSION</b>Astragalus Membranaceus Injection can inhibit the TNF-alpha-induced expression of IL-6 and sICAM-1 by suppressing NF-kappabeta activation, suggesting its protective effect on the endothelial function.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Veias Umbilicais
/
Medicamentos de Ervas Chinesas
/
Células Cultivadas
/
Química
/
Interleucina-6
/
Fator de Necrose Tumoral alfa
/
Molécula 1 de Adesão Intercelular
/
Astragalus propinquus
/
Biologia Celular
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2009
Tipo de documento:
Artigo
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