Role of bone marrow-derived mesenchymal stem cells in reduction of graft-versus-host disease by effecting CD4+CD25+ regulatory T cells in rats / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 1210-1214, 2006.
Article
em Zh
| WPRIM
| ID: wpr-282698
Biblioteca responsável:
WPRO
ABSTRACT
The study was purposed to investigate the effects and mechanism of bone marrow-derived mesenchymal stem cells (MSCs) on graft-versus-host desease (GVHD) after allogeneic hematopoietic stem cell transplantation (allo-HSCT). The model of GVHD in rat had been established by allo-HSCT with donor derived T cells. The occurence of GVHD in recipients was observed in condition with or without donor derived MSC co-transplantation. Effects of MSCs on GVHD were analyzed by model rat survival rate and pathology. Proportions of CD4+CD25+ regulatory T cells were determined by using label spleen lymphocytes and thymocytes with double fluorescent-labeled antibodies and flow cytometry. The results showed that MSCs inhibited the lethal GVHD after HSC co-transplantation and increased the survival rate. The ratio of CD4/CD8 deceased in GVHD group in different levels, as compared with that in the experimental group. The proportion of CD4+CD25+ regulatory T cells of spleen lymphocytes was 31.55 +/- 7.58% and 20.90 +/- 1.90% in experimental and GVHD groups, respectively. Similarly, the proportion of CD4+CD25+ regulatory T cells of thymocytes was 93.20 +/- 2.69% and 57.17 +/- 6.79% in experimental and the GVHD groups, respectively. Meanwhile the proportion of CD4+CD25+ regulatory T cells was higher in experimental group than that in GVHD group. It is concluded that MSCs may prevent the lethal GVHD after allo-HSC co-transplantation and raise the survival rate of model rats by acting on the CD4+CD25+ regulatory T cells in vivo.
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Assunto principal:
Fisiologia
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Ratos Endogâmicos F344
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Células da Medula Óssea
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Linfócitos T CD4-Positivos
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Transplante de Medula Óssea
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Ratos Wistar
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Linfócitos T Reguladores
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Linfócitos T CD8-Positivos
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Biologia Celular
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Alergia e Imunologia
Limite:
Animals
Idioma:
Zh
Revista:
Journal of Experimental Hematology
Ano de publicação:
2006
Tipo de documento:
Article