Feasibility of HLA-DRB1 matching by using DHPLC / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 1183-1187, 2006.
Artigo
em Chinês
| WPRIM
| ID: wpr-282704
ABSTRACT
To study feasibility of HLA-DRB1 matching by using denatured high performance liquid chromatography (DHPLC), 20 pairs of DNA samples from donors and recipients of hematopoietic cell transplantation (HCT) for DRB1 matching and 2 pairs of samples from donors and recipient of HCT for DRB1 mismatching were studied by DHPLC and PCR-SSP. After being amplified and annealed slowly to produce heteroduplex, PCR products for exon 2 of DRB1 were detected by DHPLC to find matched or mismatched peaks in chromatogram. The results showed that DHPLC and PCR-SSP were consistant with matched or mismatched HLA-DRB1 typing. The results of DHPLC and PCR-SSP for matching were compared by using kappa test (kappa = 0.776, P = 0.00), which suggested DHPLC for HLA-DRB1 matching was in agreement with PCR-SSP. In conclusion, DHPLC for HLA-DRB1 matching is economic and convenient, moreover, will not be affected by unknown genes in HLA-DRB1 locus.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Polimorfismo Genético
/
Doadores de Sangue
/
Teste de Histocompatibilidade
/
Dados de Sequência Molecular
/
Sequência de Bases
/
Antígenos HLA-DR
/
Estudos de Viabilidade
/
Cromatografia Líquida de Alta Pressão
/
Transplante de Células-Tronco Hematopoéticas
/
Alergia e Imunologia
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Experimental Hematology
Ano de publicação:
2006
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS